Association constant of the sex-specific agglutinin in the yeast, Hansenula wingei.
نویسندگان
چکیده
Association constants were determined for absorption of the sex-specific agglutinin from mating type 5 of Hansenula wingei by cells of type 21, the opposite mating type. Absorption was determined by following the radiolabeled sulfur in purified agglutinin extracted from cells grown in 35S-labeled medium. Association was proved to be reversible under conditions where cell agglutination was In sexual agglutination, cells of the two mating types of a species of yeast agglutinate on mixing. Some pairs do so vigorously (Wickerham, 1956). In Hansenula wingei NRRL2340, a species which may have strongly agglutinative mating types, the agglutinative factor from mating type 5 can be digested from the cells with proteases such as subtilisin. This factor, which we named 5-Agl for convenience, agglutinates type 21 cells, the opposite mating type, and is strongly absorbed by them. The agglutination and absorption are specific for type 21 cells of H. wingei. In this report the absorption isotherm of 5-Ag on type 21 cells are examined under various conditions to determine the association constants and free energy of association. While this information is interesting in itself, our primary purpose is to use these features of the binding reaction in future work to examine further the nature of 5-Ag binding. The 5-Ag particles are macromolecules which appear to have about six active sites for binding (Taylor and Orton, 1968). The particles have a molecular weight of about 106 and are composed of 4 %protein and the remainder, mannan. The active sites depend on the integrity of the disulfide bonds associated with them, of which there is one per site. It is possible that several of these active sites act cooperatively. In a report to be submitted, we examine the interrelation of these active sites, using the procedures developed here.
منابع مشابه
Cooperation among the active binding sites in the sex-specific agglutinin from the yeast, Hansenula wingei.
Changes in properties of 5-agglutinin were examined during the course of the reaction to inactivate it by cleaving disulfide bonds. During this reaction both agglutinative titer and apparent free energy of absorption decreased rapidly with a relatively small proportion of disulfide bonds reacted. Fractionation experiments disclosed that very little biologically inactive material appeared until ...
متن کاملSterols as Components in the Mating Reaction of Hansenula Wingei.
Hunt, D. E. (Charles V. Chapin Hospital, Providence, R.I.) and P. L. Carpenter. Sterols as components in the mating reaction of Hansenula wingei. J. Bacteriol. 86:845-847. 1963.-Agglutinative mating strains 5 and 21 of the yeast Hansenula wingei did not agglutinate after the cells of these two strains were treated with nystatin. The agglutination-inhibiting action of this antibiotic could not b...
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Hunt, D. E. (Charles V. Chapin Hospital, Providence, R.I.). Amino acids as protective compounds in ultraviolet-irradiated Hansenula wingei. J. Bacteriol. 88:893-895. 1964.-"Prefeeding" Hansenula wingei with small concentrations of either tryptophan, cystine, tyrosine, phenylalanine, leucine, cysteine, threonine, isoleucine, ethionine, norvaline, or histidine protected this yeast against inactiv...
متن کاملMating reaction in the yeast Hansenula wingei; preliminary observations and quantitation.
Wickerham (1956) has described a new species, Hansenula wingei, which exhibits a rather unusual mating reaction. When vegetative haploid cells of the two mating types are mixed, a strong agglutination reaction may occur. On agar, the cells set to a solid mass which resists further mixing and adheres tenaciously to the instrument used for mixing the cells. When the mass of cells is placed in wat...
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ورودعنوان ژورنال:
- Biochemistry
دوره 9 14 شماره
صفحات -
تاریخ انتشار 1970